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Nutrient agar (with 5% defibrinated sheep blood for aerobic incubation) and Schaedler agar (with 5% sheep blood for anaerobic incubation) were used for cultivation. You can be signed in via any or all of the methods shown below at the same time.The email address and/or password entered does not match our records, please check and try again.Login failed. This site uses cookies. Plates were incubated at 38 ± 1°C.
The roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in diarrhea in dogs. Niilo L. Clostridium perfringens in animal disease: A review of current knowledge. the site you are agreeing to our use of cookies. (not C. perfringens) were cultured from duodenal biopsies in 2 of 9 control dogs. Biotyper score values of ≥2.3 were accepted as identification on species level. This product could help youAccessing resources off campus can be a challenge. By continuing you agree to the Copyright © 2020 Elsevier B.V. or its licensors or contributors. Thus, the aim of our study was to describe in detail the distribution, character, and grade of microscopic lesions, and to investigate the etiology of AHDS. Clostridium perfringens is a commensal in the canine gastrointestinal (GI) tract (Marks et al., 2011) and responsible for a spectrum of diseases. Lean Library can solve it Within 30 min of biopsy collection, samples were plated onto agar plates using sterile tweezers, followed by 3-phase streaking using an inoculation loop for semi-quantification. If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Endoscopic biopsies of the gastrointestinal tract were taken and examined histologically (H&E, Giemsa), immunohistochemically (Acute hemorrhagic diarrhea syndrome (AHDS) is a common cause of hemorrhagic diarrhea in dogs.To date, the etiology of AHDS is unconfirmed. After blocking with normal serum for 30 min, slides were incubated with the primary antibody for 60 min. Sign in here to access free tools such as favourites and alerts, or to access personal subscriptionsIf you have access to journal content via a university, library or employer, sign in hereResearch off-campus without worrying about access issues.
In previous reports, distribution of lesions has been variable: small and large intestine except very proximal and distal portionsInterestingly, significant differences in lesion severity were observed between animals.
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This analysis was conducted twice, both as intention-to-treat analysis with the last value carried forward and as a per-protocol analysis excluding the animals that did not have histologic scores from every intestinal region.Gastric biopsies were available from 10 dogs (4–9 biopsies per animal, Duodenal biopsies were available from 10 dogs (8–15 biopsies per animal, Ileal biopsies were available from 8 dogs (2–9 biopsies per animal, Biopsies of the colon were available from 9 dogs (2–10 biopsies per animal, There was no significant difference in the necrosis scores of the duodenum, ileum, and colon with either per-protocol or intention-to-treat analysis (Parvoviral antigen could not be demonstrated in the small intestine in any of the cases.Bacterial culture of duodenal biopsies revealed growth of bacteria in 8 of 9 dogs with AHDS and no bacterial growth in 1 dog with AHDS. Introduction. Lesions were detectable in the entire intestinal tract with variable individual distribution.
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